Sentence examples for maintenance of plasmids from inspiring English sources

For the selection and maintenance of plasmids, the culture medium was supplemented with 50 μg L−1 of kanamycin.

Escherichia coli strain JM109 was used for routine bacterial transformations and maintenance of plasmids.

The introduction and maintenance of plasmids in cells is often associated with a reduction of growth rate.

Transformed E. coli cells were grown in LB medium supplemented with 100 µg/mL of ampicillin (Sigma-Aldrich, St . Louis MO) for selection and maintenance of plasmids.

Cultures were grown at 30°C in synthetic complete (SC) media with 2% raffinose, lacking one or more amino acids for maintenance of plasmids.

These results suggest that acquisition and maintenance of plasmids may represent a major mechanism for pseudomonads to change their genetic composition of the genome and even acquire new virulence factors [53].

For maintenance of plasmid, kanamycin (50 μg/mL) or ampicilin (100 μg/mL) were added.

The use of two antibiotics (ampicillin, chloramphenicol) allowed the stable maintenance of plasmid in the recombinant bacterium.

E. coli M15[pREP4] harbouring plasmid pQE-30-LE::cel5A displayed endoglucanase-specific activity, while maintenance of plasmid pQE-30-LE::bgl1 resulted in activity towards esculin.

Often, however, it is difficult to discern long-term expression resulting from transposition vs nonhomologous chromosomal recombination or maintenance of plasmid DNA in an extrachromosomal form.

The interaction between the two types of cheating is important for the maintenance of plasmid diversity along both the axes.