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The establishment and maintenance of interaction patterns increasingly more complex depend, to some extent, on the availability and involvement of a third person (Bronfenbrenner 2001).
Maintenance of interaction of RNase II with the RNaseE that lack the membrane-binding domain does not exclude a role of membrane-association of the proteins in the RNase II RNaseE interaction.
In these experiments, a fraction of membrane-localized GFP-Lpd850−1250aa remained at the leading edge, presumably due to the maintenance of interaction with Ena/VASP and/or Abi1/endophilin.
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Also, the active site is as well organized in the structures containing lesions as in the unmodified control; key properties include maintenance of the octahedral coordination of the Mg2+ ions, the in-line O3′ Pα attack distance, maintenance of interactions with key amino acid residues, and preservation of the two water molecules participating in the WMSA mechanism.
To determine the kinetics of the maintenance of the interaction of the peptide-MHC complex, we repeated the experiment of adding the photo-reactive peptide to the T2 cells followed by UV exposure.
Thus, we developed alternative techniques to evaluate, in tumors, the expression status of BRCA1 at three different levels: mRNA, protein, and maintenance of BRCA1 interaction with BARD1.
These strongly up-regulated transcripts we identified are probably connected to recognition events and maintenance of the interaction with AcH 505.
We reasoned that the probability of the yeast interaction being conserved in humans would be higher for those proteins that show homology in another higher eukaryote because this would be evidence for the maintenance of the interaction in the Metazoan lineage.
Unlike acute infections, persistent infections are difficult to treat owing to their complex biology which involves host-pathogen interactions at various levels ranging from molecular to population levels and maintenance of such interactions for prolonged time adds further complexity [19].
Modification of kanamycin A functional groups that have been directly implicated in the maintenance of specific interactions with RNA led to a decrease in affinity for the target RNA.
This might due to the finding that, at elevated temperature, Arg exhibits more stable maintenance of electrostatic interactions and a net positive charge, since the pKa of Arg side chains are more basic (12.1) than Lys (11.1) (pKa values drop as the temperature increase).
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