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Also included among its concerns are major flight-vehicle subsystems such as propulsion and avionics (aviation electronics) and key support systems necessary for the testing, operation, and maintenance of flight vehicles.
Maintenance of flight bouts was rescued significantly upon increasing neuronal activity by expression of NaChBac.
DOI: http://dx.doi.org/10.7554/eLife.07046.032 10.7554/eLiFigure46.033 Figure 7 figure supplement 5. Maintenance of flight requires Fz2/Ca2+ signaling in dopaminergic neurons.
DOI: http://dx.doi.org/10.7554/eLife.07046.031 10.7554/eLiFigure46.032 Figure 7 figure supplement 4. Maintenance of flight requires dFz2/Ca2+ signaling in dopaminergic neurons.
The study demonstrates that dFz2 regulates maturation of a subpopulation of DA neurons called PAM neurons and that these neurons are critical for maintenance of flight.
Knockdown or inhibition of Fz2/Ca2+ signaling during maturation of the flight circuit in pupae reduces Tyrosine Hydroxylase (TH) expression in the PAM neurons and affects maintenance of flight.
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Expression of Go-IR and PTX.16 in dopaminergic neurons reduced both flight times, and the maintenance time of flight patterns recorded from the DLMs.
These data support a requirement for post-synaptic dopamine receptors in MB neurons that function for maintenance of acute flight.
Any single gene or combination of genes within the 40 could be essential for the initiation and/or maintenance of directional flight in migrants.
These data support a role for the PAM-MB circuit in maintenance of long flight bouts through dopaminergic synapses on α' β' MB lobes.
Data from the earlier version of the manuscript with c305aGAL4 is now shown in Figure 7 figure supplement 2. The Results have been modified accordingly (please see the subsection entitled "Maintenance of acute flight requires synaptic activity in α' β' lobes of the mushroom body").
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