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In mouse cell culture models, YAP expression is associated with the maintenance of embryonic stem cells, and the reduction of nuclear YAP corresponds with differentiation [42, 57].
They have also been implicated in the maintenance of embryonic and somatic stem cells [12].
In particular, it is well known that SOX2 plays important roles in maintenance of embryonic stem (ES) cell self-renewal and pluripotency [4], [5].
In Zebrafish, miR-430 [20] has been shown to silence maternal RNAs, miR-214 is involved in proper somite specification [21] and miR-375 is necessary for the maintenance of embryonic pancreas integrity [22].
We hypothesize that P4 in the oviparous CAM might be expected to serve similar roles as in eutherians contributing to the maintenance of embryonic development, timing of hatch, and growth of the embryo and of the CAM.
The notion that the determination and maintenance of embryonic stem (ES) cell pluripotency and self-renewal is related to an epigenetic state characterized by an open chromatin conformation has received considerable support over the last several years [1] [6].
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To exclude non-specific binding, we used the differentiated ES (dES) cells and primary mouse embryonic fibroblast (PMEF) cells, a feeder layer for the maintenance pluripotency of embryonic stem cells, as a control.
B-Myb is therefore critical not only for mouse embryonic development, but also for a normal karyotype and maintenance of viable embryonic, and potentially, all proliferating somatic stem cells.
Hedgehog (Hh) signaling is crucial for the generation and maintenance of both embryonic and adult stem cells, thereby regulating development and tissue homeostasis.
Here, we investigated whether PAPST-dependent sulfation is involved in regulating signaling pathways required for the maintenance of mouse embryonic stem cells (mESCs), differentiation into the three germ layers, and neurogenesis.
Generation and maintenance of the embryonic cells has been described previously [ 21].
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