In contrast, COUP-TFII is not essential for the maintenance of Leydig cell function as shown by deletion of COUP-TFII in adult male mice.
Since COUP-TFII is also expressed in mature Leydig cells in the adult, we asked whether COUP-TFII is also essential for the maintenance of Leydig cell function.
In contrast, when COUP-TFII was deleted after Leydig cell differentiation was complete, no discernible phenotype was detected, suggesting that COUP-TFII is not important for the maintenance of Leydig cell function and male reproduction in the adult.
Based on these results, we conclude that COUP-TFII is not essential for the maintenance of Leydig cell function and male fertility in adult under normal conditions.
Consistent with these findings, western blot confirmed that COUP-TFII was not essential for the maintenance of Leydig cell marker genes expression in adults (Fig. 7B and 7C).
Taken together, these results indicate that COUP-TFII plays a major role in differentiation, but not the maintenance of Leydig cells.
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34 COUP-TFII has previously been shown to initiate PROX1 transcription, and although not necessary for the maintenance of expression, may potentially be initiating PROX1 expression in AVMs.
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