Thus, maintenance of nucleotide pools is a critical challenge for starving Kras-driven tumor cells.
Genes whose functions are related to DNA maintenance and nucleotide salvage pathway are highlighted in bold.
The existence of a binding site upstream of edd would furthermore allow a control of the metabolic flux into the pentose-phosphate-pathway. Fis is a small protein that plays an important role in the organization and maintenance of nucleotide structure by binding to DNA.
These were the bacterial histone-like HU protein involved in DNA compaction, the XthA protein, related to the organization and maintenance of nucleotide structure, the PriB protein, involved in replication, and the Fis protein (Fig 2 and additional file 4, 1B, 1C).
N order to determine the selective pressure on the maintenance of the nucleotide sequences, the number of nonsynonymous nucleotide substitution per nonsynonymous site (dN) and the number of synonymous substitutions per synonymous site (dS) were determined for N, A and B exons.
In summary, the UMP/CMP kinase (EC 2.7.4.14) is not critical for the phosphorylation of CMP, dCMP and maintenance of natural nucleotide pools in cells.
The UMP/CMP kinase (EC 2.7.4.14) expressed in RKO cells is not critical for the phosphorylation of (d CMP and the maintenance of natural nucleotide pools.
The role of this protein is considered critical for the maintenance of pyrimidine nucleotide pool profile and for the metabolism of pyrimidine analogs in cells, based on the in vitro study of partially purified enzyme and recombinant protein.
The yield of moderately and strongly nuclear staining of fosfo-ATMSer1981 was found to be dependent on the disturbance of the de novo maintenance of intracellular nucleotide pools.
Therefore, metformin's ability to alter the carbon flow through the folate-related de novo maintenance of intracellular nucleotide pools may represent a major mechanism of metformin's anticancer effect at physiological folate substrate concentrations and metformin blood serum levels.
