Sentence examples for maintenance medium was from inspiring English sources

The mESC maintenance medium was used for spontaneous differentiation without supplementing LIF, the EsK3β inhibitor or the MEK inhibitor.

The maintenance medium was added to induce hydrogel formation.

Fresh maintenance medium was added and plates were returned to the incubator.

The maintenance medium was changed every 48 h until the cells were utilized for experimentation (9 days from the initiation of differentiation).

This basal maintenance medium was replenished every other day during the remainder of the culture experiments, which lasted 4 to 10 additional days (8 to 14 days following the addition of the first induction medium).

Maintenance medium was composed of DMEM (Gibco, Invitrogen, NY, USA) supplemented with 1 mM MEM Sodium Pyruvate (Gibco, Invitrogen, NY, USA), 1% Penicillin-streptomycin-nystatin solution (Biological Industries, Israel), 10% heat inactivated FBS (Hyclone, USA), and 10 µg/ml insulin (Sigma, MO, USA).

The constituents of the maintenance medium were the same as those of the growth medium except that only 2 % FBS was added.

Two hundred microliters of maintenance medium were added to the negative control wells (cells without virus or treatment).

The ATDC5 cells cultured on the PAMPS and PDMAAm gels in the maintenance medium were compared with the cells cultured on the polystyrene (PS) dish in the maintenance medium (the negative control) and in the differentiation medium (positive control).

Standard BG1Luc cell culture maintenance medium is complete growth medium containing phenol red, FBS and various additives and thus contains estrogen and chemicals with EA that facilitate cell growth.

Later, serial 2-fold dilutions of MBS 10 to 0.31 mg/ml prepared in RPMI-1640 mediumnance medium were added to the cells, in triplicates, to a final volume of 200 μl/well.