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The recombinant product was primarily transformed in maintenance host E. coli BL21 (DE3).
Wild boar (Sus scrofa) recently emerged as a major maintenance host for TB in some European countries.
Cattle are the maintenance host for Leptospira borgpetersenii serovar Hardjo (subtype hardjobovis) and Leptospira interrogans serovar Hardjo (subtype hardjoprajitno), which are serologically indistinguishable but genetically distinct [10].
There is evidence that the wild boar is a maintenance host for M. bovis in the Iberian Peninsula [4], where wildlife TB is re-emerging [5], [6].
Typically, long term colonization by pathogenic Leptospira of the proximal renal tubules of mammalian maintenance host species provides a persistent source of infection; thus, pathogenic Leptospira is shed in the urine of chronically infected animals, facilitating transmission to naïve hosts [2].
In-depth assessments are then necessary to understand lifecycles and drivers of reservoir (maintenance host) populations.
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One way to identify potential maintenance hosts is to estimate key parameters associated with transmission and pathogenicity.
Wild terrestrial birds can act as potential local spreaders or bridge hosts for avian influenza viruses (AIVs) between waterfowl (the maintenance hosts of AIVs) and domestic avian populations in which AIVs may cause disease.
When assessing the potential of wild and feral animal species at a locality to act as maintenance hosts of FMD virus, estimates of the population size and distribution might serve as a useful indicator of potential outbreaks in some circumstances.
Here we assess the potential for three native rodent species of the Brazilian Pantanal (Clyomys laticeps, Thrichomys pachyurus and Oecomys mamorae) to act as reservoir or maintenance hosts of Trypanosoma evansi, an important parasite of domestic livestock.
We investigated how the size and distribution of wild deer and feral pigs – species that might act as potential foot-and-mouth disease (FMD) virus maintenance hosts – might affect the persistence and spread of FMD.
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