As the plane approached Little Rock, Mr. Davis remarked, half-jokingly: "Jay is one of the highest-maintenance candidates I've ever had.
The list of Xi-maintenance candidate factors from these prior studies is distinct from ours, with the exception of Dnmt1, for several potential reasons.
PCR-based molecular screening methods are a universal option and have been demonstrated with CRISPR/Cas9-mediated NHEJ, but the associated generation and maintenance of candidate fly stocks is time and labor intensive (Yu et al. 2013).
Since genome-wide RNAi screens used for discovering functional genes in stem-cell self-renewal and pluripotency maintenance produce candidate lists that are inherently noisy, the SVM-based classifier can be applied to prioritize experimental choices when facing with a large list of candidate genes to verify and further functionally characterize.
Although the key soluble and/or cellular components which contribute to the tumor heterogeneity and/or maintenance of the candidate CSC in MPE-primary cultures have yet to be defined, it is important to note that cells bearing surrogate labels for cancer stem cells are included in the MPE-tumor mix.
Here, we studied ATF7IP (MCaF1), a protein previously characterized to coordinate DNA methylation and histone H3K9 methylation through interactions with the methyl-DNA binding protein MBD1 and the histone H3K9 methyltransferase SETDB1, as a candidate maintenance factor of the Xi.
Debate also continues regarding the appropriate selection of candidates for maintenance treatment [ 39].
As shown in the current study, ideal candidates for maintenance may be potentially identified by symptom burden or ECOG PS.
However, those with mCRC who cannot tolerate the toxicity of continuous combination therapy alone or in combination with targeted drugs are candidates for maintenance treatment.
Several significant deviations from the neutral expectation were detected by either analysis, the negative values of Tajima's D and dN/dS ratios smaller than 1 suggested purifying selection, especially on genes from the meristem maintenance compartment where six candidates showed significant D value (Table 3 and Additional file 3).
In routine clinical practice, patients with high tumor burden and symptomatic disease may be candidates for maintenance (immediate) therapy, whereas asymptomatic patients are often given a 'drug holiday' after achieving maximum response, followed by second-line (delayed) treatment after progression [ 22– 25].
