Sentence examples for maintenance assay from inspiring English sources

To determine whether the DsRed-Express2 derivatives retain low cytotoxicity in mammalian cells, a fluorescence maintenance assay was performed [ 5].

In the PRE maintenance assay, DNA fragments are tested for their abilities to limit enhancer-driven expression of a reporter gene to the normal expression domains of the endogenous gene of the enhancer.

Curiously, although the gt 4 fragment overlaps with gt 5 and behaves as a PRE in the maintenance assay, only one out of nine SD10- gt4 lines exhibited pairing-sensitive silencing.

Consistent with the presence or lack of PREs based on the PRE maintenance assay, three out of four SD10- gt1 and four out of five SD10- gt5 lines demonstrated pairing-sensitive silencing compared to zero out of three SD10- gt 2 and zero out of five SD10- gt3 lines.

However, hermaphrodites in the repeated mating experiment were exposed to "new" virgin males every day (most likely with high mating efficiency), whereas hermaphrodites in the male maintenance assay encounter the same males over consecutive days, which are likely to show reduced mating efficiency over time (due to the likely high energy demand per mating as well as sperm depletion).

To understand whether there was a contribution of Hat1p/Hat2p to the function of a specific origin (ARS1), we made use of a plasmid-based minichromosome maintenance assay, which is often used to characterize mutations that affect the efficiency of initiation of DNA replication [ 45].

The function of MRN proteins at DS was investigated using the DNA replication and plasmid maintenance assays.

We used plasmid maintenance assays in S. cerevisae [26], [33], to provide functional evidence that ACS-containing NUMTs act as origins of replication.

Moreover, to assess the ability of lysine 37 mutant strains to carry out replication, plasmid maintenance assays were completed, where the ability of a cell to replicate a reporter plasmid containing a single origin of replication and a selectable marker is measured [84].

However, not all DNA fragments that function as PREs in maintenance assays also produce pairing-sensitive silencing.

All male maintenance assays were done on 9 cm NGM plates, seeded with 1 ml of E. coli OP50 culture.