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When producing plasmid DNA in Escherichia coli, a number of biological restraints, triggered by plasmid maintenance and replication as well as culture conditions are responsible for limiting final biomass and product yields.
By taking advantage of the highly efficient yeast in vivo homologous recombination mechanism, this method synthesizes the entire expression vector containing the target biosynthetic pathway and the genetic elements needed for DNA maintenance and replication in individual hosts in a single-step manner.
In addition, these chromatin "marks" serve as signals to other specialized proteins involved in chromatin organization, gene transcription, genome maintenance, and replication (Kouzarides, 2007; Sharma et al., 2010).
The 26 kb plasmid of R. peacockii (pRPR) contains 20 putative genes (Table 1), two of which are involved in plasmid maintenance and replication, ParA and DnaA.
[26] Particularly, EBNA1 expression is essential for viral episome maintenance and replication and it is expressed in all known forms of latency.
In addition to functions in cellular maintenance and replication [3], telomeres are also thought to be a defense mechanism against cancer, as short telomeres induce senescence and thus inhibit uncontrolled cell proliferation [4].
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In the ex-post evaluation, the percentage of families actively involved in both maintenance and replications of SWC practices was assessed by means of a field survey.
The average investment in these villages in maintenance and replications of SWC practices was estimated to be 20 labor days per family during these two years (2001 to 2003).
miR-2940-5p, whishighlyinducednduced in Wolbachia-infected Ae. aegypti, was previously reported to enhance Wolbachia efficient maintenance and limit replication of DENV in Ae. aegypti [ 13].
We find that genes associated with GO terms representing chromosomal maintenance and DNA replication are overrepresented within our list of 102 hits (Table 4), indicating these cellular processes are likely to be important for regulating the stationary phase stability of the ade2 -min3 minisatellite.
Even the simplest members of the three modern domains of life use DNA to record their "recipes" and a complex array of RNA and protein molecules to "read" these instructions and use them for growth, maintenance and self-replication.
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maintenance and repeat
maintenance and advertising
maintenance and improvement
maintenance and desilting
maintenance and packing
maintenance and capital
maintenance and restoration
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maintenance and mortgage
maintenance and renewal
maintenance and demolition
maintenance and safety
maintenance and infection
maintenance and rental
maintenance and expansion
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