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Thus, telomerization of hMSC by hTERT overexpression maintains the stem cell phenotype of hMSC and it may be a useful tool for obtaining enough number of cells with a stable phenotype for mechanistic studies of cell differentiation and for tissue engineering protocols.
LIF, a pleiotropic cytokine, maintains the stem state of MSCs [ 14] and other stem cells [ 15].
One daughter maintains the stem cell properties, while the other differentiates to replenish specialized cell types.
The distal maximum in the root tip maintains the stem cell niche.
Similar to the shoot meristem, the root QC maintains the stem state of the surrounding cells and prevents these cells from differentiating [ 10].
However, Seidel and Kimble found that the germline quiescence caused by starvation maintains the stem cell state even when Notch signaling is prevented.
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These self-renewing cancer stem cells (CSCs) or tumor-initiating cells (TICs) must not to be confused with normal adult stem cells, which are pluoripotent organ-specific cells that have the biological properties of self-renewal and with each division they divided both progenitor cells and at least one offspring that maintains the stem-cell phenotype.
Therefore, the number of CB hematopoietic stem/progenitor cells (HSPCs) should be increased while maintaining the stem cell characteristics.
Stem cells are in close contact with a special microenvironment, called the stem cell niche, which is crucial for maintaining the stem cell identity and the potential to self-renew (Li & Xie 2005).
However, the therapeutic potential of hMSCs cannot be realized without gaining a more complete understanding of the in vitro culture parameters that can best maintain the stem cell phenotype and multipotency during expansion.
NSCs proliferated and maintained the stem cell state, validated by Nestin staining (Figure 3C).
More suggestions(15)
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