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We herein demonstrate that by polarizing 13C with photo-induced radicals, which can be subsequently annihilated using a thermalization process that maintains the sample temperature below its melting point, hyperpolarized 13C-substrates can be extracted from the DNP apparatus in the solid form, while maintaining the enhanced 13C polarization.
The microtome is housed in a freezer unit that maintains the sample at –80°C during sample slicing and image acquisition.
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It can accommodate a 96-well-plate or Eppendorf tubes and maintains the samples at 0°C.
After maintaining the sample at this temperature for 10 30 min, it was annealed at T = 570 K for 5 min to induce cyclodehydrogenation reactions.
Furthermore, maintaining the sample in tension throughout the process prevents it from becoming deformed or breaking during the heat treatment.
Compressed gas is used to maintain the sample at seafloor pressure before and during sample withdrawal onboard ship, allowing subsampling without degassing the fluid remaining in the sampler.
The new algorithms can be used to sample from a relational database as well as data streams, with the ability to examine each transaction only once, and maintain the sample on-the-fly in a streaming fashion.
Test imaging of dodecane showed that 0.5 2 h were needed to obtain one 3D image; thus, we developed a specimen-cooling system to maintain the sample at a constant temperature.
To avoid the variation of the temperature, a cooling system was employed to maintain the sample temperature at about 25°C.
However, to maintain the sample size and integrity of the overall data results, these scores were included in the statistical analysis.
Correspondingly, in order for us to maintain the sample integrity and quality over time in large scale biobanking, we apply the principle of single usage [17].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com