Thus, the barrier element has two functions: (1) maintaining stable expression, and (2) maintaining active chromatin modifications.
Thus, the distal 650 bp segment between the Sac and Bam sites is not sufficient to maintain stable expression.
In animal studies, upregulation of ΔFosB, a product of the Fosb gene induced by chronic administration of virtually all drugs of abuse was found to persists for several weeks and may maintain stable expression of genes associated with drug addiction [73], [74].
They can maintain stable expression of exogenous genes, even during the process of proliferation and differentiation.
In endosperms, the expression level of TaLCYB was reduced, TaHYD1 and TaLCYE were up-regulated, TaHYD2 maintained stable expression, and TaPSY was slightly down-regulated.
Significantly, we revealed that 98.1%, 97.9, and 99.4% of genes maintained stable expression in human Sertoli cells at P5/P1, P10/P1, P10/P5, respectively.
For example, the expression of TaHYD1, which is more related with β,β-branch synthesis, was up-regulated in the transgenic lines while the expression of TaHYD2, which is more related with β,ε-branch synthesis, maintained stable expression.
As RNA polymerase-III expression systems can offer a great potency for maintaining stable expression of short RNA molecules in vitro as well as in vivo [ 20], the U6-driven expression vector Pgensil-1 was applied in this study.
The self-regulation mediated through RAS small GTPase regulatory genes may represent the fastest means of regulating SOD3 expression, which is required to maintain stable expression of the mRNA production and the redox balance in tissues.
Real-time bioluminescence imaging of mouse hes1 dynamics for 15 hours showed that dissected PSM fragments are capable of maintaining stable expression but gradually become out-of-phase [ 49].
