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This resource currently maintains samples and associated phenotypic data from 2332 MND subjects and 4692 controls.
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For decades, researchers have used embedding media such as hydrogels to maintain samples in place.
Lower porosity and impurity levels may make it difficult to produce and maintain samples with the smallest nanocrystalline grain sizes.
Marine sediments retrieved with pressure coring systems such as those used by the Integrated Ocean Drilling Program maintain samples at in situ hydrostatic pressure throughout recovery.
For all experiments, a stage-top incubator (Tokai-Hit, Japan) was used to maintain samples at 37C.
It is also a non-hardening medium that can maintain samples for up to several weeks at 4°C.
Blood was collected into EDTA-containing tubes and immediately placed in IsoRack cool packs (Brinkmann Instruments, Westbury, NY) designed to maintain samples at 0°C for > 6 hr.
It is essential to grind the tissue as finely as possible, maintaining samples as cold as possible during grinding to avoid degradation.
Blood was collected into heparin-containing Vacutainer tubes, which were placed in IsoRack/IsoPack cool packs (Brinkmann Instruments, Westbury, NY) designed to maintain samples at 0°C for 6 hr.
Capture also occurred in autumn (September to November) to maintain sample size from dropped collars or fatalities.
Long term immersion tests showed that the coating was effective in maintaining sample bulk integrity for the duration of the 30 day tests.
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