Sentence examples for maintaining wild type from inspiring English sources
Amongst a collection of 28 strains carrying deletions in genes for glycosylphosphatidyl inositol (GPI -anchored proteins, the Δsed1 and Δtos6 strains had siGPI -anchoredigher BGL-activity whilst maintaining wild typroteinsh.
Specifically, the impact of accumulating abasic sites was examined by (i) increased expression of Mag while maintaining wild type levels of Apn1 (WT/pMAG), (ii) maintaining wild type levels of Mag in an Apn1 deficient strain (Δapn1), or (iii) increased expression of Mag in an Apn1 deficient strain (Δapn1/pMAG).
The Z. tritici ku70 and ku80 null strains facilitate increased HR mediated gene targeting while maintaining wild type growth and pathogenicity in planta.
Surprisingly, positive supercharging maintained wild type activity levels in ILs, while negative supercharging drastically reduced activity.
The vector we employed encodes a fusion protein (GFPBcl-xL) previously shown to maintain wild type Bcl-xL biological function [38].
Our strategy renders mice heterozygous at the RIα locus, and expression from a single wild-type RIα allele appears to be adequate to maintain wild type PKA activity levels in most tissues.
In contrast to random PEGylation of the enzyme by NHS-ester mediated chemistry, controlled mono-PEGylation of A1-III alginate lyase produced a conjugate that maintained wild type levels of activity towards a model substrate.
To ask whether this Ca2+ conducting channel was responsible for calpain activation and the migration of T cells, we used a human T cell line derived from a immunodeficient patient homozygous for a non-functional mutated ORAI1-R91W protein and compared its migratory potential with a similarly maintained wild type control T cell line [34].
No measurable changes were observed in kinase activity from ES cells harboring the RIαB allele compared to WT ES cells, suggesting that a single allele is sufficient to maintain wild type levels of PKA activity in ES cells (Figure 2C, RIαB lane).
YPD medium (1% yeast extract, 2% peptone and 2% D-glucose) was used to routinely maintain wild type strain.
Despite the dramatic apoplastic bypass in sgn3 roots, the mutants managed to maintain wild type levels of sodium (Na), calcium (Ca), or boron (B), for example.
