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Survival requires maintaining transcript levels of essential genes above a threshold value.
The Ovation RNA-Seq V2 kit is able to amplify small quantities of RNA from tissue samples while maintaining transcript levels nearly identical to those from matched unamplified input RNA, demonstrating an overlap of 93% when comparing each matched pair individually.
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How the transcriptional regulatory machinery precisely controls and maintains proper transcript levels is not well understood.
These results suggest that MUC16 is required to maintain normal transcript levels of Muc1.
Normal plants in T1 35showedsa4 showed a two-fold transgene up-regulation and maintained endogenous transcript levels similar to those in control plants (Table 1).
However, our finding that these were disproportionately NOVA-mediated actions to maintain neuronal transcript levels (215 vs 14) is consistent with an overall action linking alternative splicing suppression of NMD exons to steady-state transcript levels.
In EPI−/− epidermis treated with acetone, transcripts related to innate immunity (in particular RAGE, S100A8, S100A9 and IL-1β) were higher than in WT, and these differences were maintained when transcript levels were upregulated by TPA treatment.
These authors found that, at least in these two species, duplicate copies of genes are more likely to be retained if the expression level is high than if it is low, presumably in order to maintain high transcript levels.
Global profiling of gene expression kinetics with samples from TPA-treated wt mice revealed a comprehensive list of differentially expressed genes that were strongly induced or repressed within 6 hours and maintained altered transcript levels for at least 24 hours.
In our analysis, we found that while several histone chaperones maintain their transcript levels, either high or low, throughout development and across different tissues, many others are expressed at a higher level at a particular developmental stage or in specific plant tissues.
Stress-response genes also maintained low transcript level during the experiment.
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