Chondrocytes demonstrated viability, proliferation and migration within the matrix, while maintaining their phenotype, as seen by expression of collagen type II and aggrecan, and functionality, as seen by enhanced glycosoaminoglycan (GAG) deposition with time.
NCs in clusters were (co cultured in three-dimensions, in vitro, under physiological hypoxia and osmolarity, with the aim of maintaining their phenotype [ 24, 37- 40].
hASCs can be differentiated into functional hECs without the use of animal supplements and are capable of reendothelizing a decellularized rat aorta while maintaining their phenotype.
This barrier function is performed by the capillary endothelial cells, the cellular environment of which is essential in establishing and maintaining their phenotype.
3D condition is able to mimic naturally microenvironment of chondrocytes in cartilage tissue, which promotes cell-cell and cell-matrix interactions and enforce a rounded chondrocytic cell shape, thus maintaining their phenotype [ 5, 13].
The extended CD8+ T cell responses were sustained over 8 weeks in the recipients, even in the absence of antigen, maintaining their phenotype (CD44hi, CD62Llo, CD27lo, and CD127lo) and distributing to tissues such as liver and lung (data not shown).
Although this method might not be the utmost technical experimental design, it was our aim to show interaction of the carbohydrate portion of AsGM1 with sialic acid residues on intact cells, displaying their specific features and properties, and thus maintaining their phenotypes as previously published [ 18– 20].
Of the 640 phenotypic knockdowns, 264 maintained their phenotype in three of four trials (Table S1).
Subsequently, we cannot confirm that BMSCs maintained their phenotype through until completion of experiments.
Results: Myometrial smooth muscle cells maintained their phenotype for at least 7 days in culture.
The MG63 cells also maintained their phenotype under conditions that support a round cell shape.
