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To obtain a clean result, we need to knock down endogenous Ubl4A while maintaining the expression of ectopically expressed Ubl4A at a level comparable to that of the endogenous protein.
When cultured on dermal fibroblasts, the cord epithelial cells were able to differentiate in vitro into a stratified multilayered epithelium expressing molecules characteristic of keratinocyte differentiation after exposure to air, and maintaining the expression of keratins K18 and K19, typical of the umbilical cord epithelium.
The results showed that elongated morphology (Group C) could help in vitro passaged tenocytes to retain their phenotype and function by maintaining the expression of tenomodulin (tenocyte marker) and collagen I (functional molecule).
Thus, LRP5 is important for stimulating or maintaining the expression of Slc38a5 gene in Müller cells.
Although we cannot exclude that the slow/oxidative phenotype of mutants could be related to a physiological adaptation of muscles to the premature aging, our data support a role for SRF in maintaining the expression of the MyHC IIB gene.
By contrast, in lineages with type B PROS expression, the LOF of pros seems to cause primarily a change in the fate of the putative GMC that behaves like a NB maintaining the expression of asymmetric division genes (such as MIRA) and overproliferating, to yield a type B clone composed of multiple large NB like cells (Fig. 7A,B).
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The induced clusters lost their expression of the cholangiocyte marker KRT7 while maintained the expression of AFP and began to express ALB (Figure 5B and C), which was only faintly expressed in hepatic progenitor cells.
Our previous studies have shown that the MKK1/2-ERK1/2 MKK1/2-ERK1/2y maintainsignalexpathwayn of Rad51 in NSCLC cells.
Immunohistochemical staining revealed cells secreted type II collagen, produced glycosaminoglycans (proved by alcian blue stain), and maintained the expression of S-100.
This indicates that compensation by the rhl QS system could override this hierarchy and maintain the expression of QS-dependent virulence factors in spite of a non-functional central las system.
Monocytes cultured with GM-CSF and IL-4 for 4 days exhibit markers characteristic of iDC and maintain the expression of both formyl peptide receptors FPR and FPRL1, as well as CCR5.
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