P. falciparum D10 cultures were maintained, synchronized, and lysed, as previously described.
Although there is no sleep delay, the disadvantage of this approach is that each node in the network needs to be synchronized, while maintaining synchronization in large-scale networks requires a great deal of communication overhead.
Interestingly, the cells growing with continuously available but low glucose continued to show steady expression of the OX and the RC phase reporters for over 20 hours, and maintained fairly synchronized cell division (Figure 5).
C. elegans [N2 wild type, DR subclone of CB original (Tcl pattern I), obtained from Caenorhabditis Genetics Center] were maintained in synchronized cultures grown in CeHR medium (see below).
To overcome this problem, we adopt a simple algorithm that maintains synchronization of working-cycles of nodes in a cluster without synchronizing the nodes' clocks.
Moreover after serum shock, GSK-3beta-mediated stabilization of Rev-erb alpha plays a key role to initiate, maintain and synchronize CRs.
Sole application of organic sources cannot maintain and synchronize the required nutrient supply to the growing plant due to lesser quantity of mineral nutrients or time needed for their mineralization to release nutrients for plant uptake (Akhtar et al. 2011).
To avoid instabilities in the HO process, a hysteresis region can be maintained by synchronizing changes in both directions of the adjacency (i.e., if the margin from cell i to j is increased by + X dB, the margin from j to i is reduced by −X dB).
In [29], Ergen et al. proposed the WTRP (Wireless Token Ring Protocol), which is a MAC protocol that exchanges special tokens and uses multiple timers to maintain the nodes synchronized.
