Sentence examples for maintaining standard tissue from inspiring English sources

Cells co-transfected with the redox reporter (roGFP) and ChaC1-mCherry were analyzed by laser-scanning confocal microscopy system (510 Meta; Carl Zeiss) with a Plan-Apo chromat 63× oil immersion lens (NA 1.4), coupled to a microscope incubator, maintaining standard tissue culture conditions (Okolab).

FLIM experiments were performed on a modified version of a previously-described laser scanning multiparametric imaging system [ 31], coupled to a microscope incubator, maintaining standard tissue culture conditions (Okolab, Pozzuoli, NA, Italy), using a pulsed (sub 10 ps, 20 MHz) supercontinuum (430 to 2,000 nm) light source (SC 450, Fianium Ltd., Southampton, UK).

Zeiss 510 Meta laser scanning confocal system with Plan-Apochromat ×63 oil immersion lens (NA=1.6) coupled to a microscope incubator that maintained standard tissue culture conditions (Okolab), was used for live cell imaging.

Both cell lines were maintained using standard tissue culture protocols.

The SW480 cell line was sorted into CD133+ and CD133− populations which were maintained in standard tissue culture conditions and underwent regular analysis by flow cytometry.

Mouse brain endothelial cells (MBEC), CRL-5904 cells (human non-small cell lung cancer from a brain metastasis), BT-474 cells (breast cancer cell line) and MDA-MB-435 (breast cancer cell line) were obtained from the American Type Culture Collection (ATCC, Manassas, VA), and were maintained in standard tissue culture conditions.

All cell lines were maintained under standard tissue culture conditions.

All cell lines were cultured at 37°C in a humidified atmosphere with 5% CO2 and maintained using standard tissue culture techniques.

These characteristics included that MSCs should be adherent to plastic when maintained in standard tissue culture conditions, be able to differentiate into osteoblasts, chondroblasts, and adipocytes, and also express or be deficient in a set of panel markers [ 32].

Mouse NIH 3T3 and human 293-EcR cells were obtained from the American Type Culture Collection and Invitrogen, respectively, and were maintained in standard tissue culture media (Dulbecco's Modified Eagle's Medium) containing 10% fetal calf serum.

To maintain the standard tissue culture condition for ECs in the flow chamber, we placed all the components, except the syringe pump, in a chamber (Chamlide TC, Live Cell Instrument, Korea) in which the temperature and CO2 concentration were controlled.