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Replace medium with fresh culture medium supplemented with 5.5 mM glucose for the all islets, maintaining separated the batches of islets according to the previous glucose concentration (during the sensitization period).
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So maintaining separate trading operations in lots of exchanges represents hugely expensive, wasteful duplication.
Or are they risk-averse, maintaining separate households and seeing one another on the odd weekend?
For a spell, different companies ran the clockwise and anti-clockwise trains on the Circle Line, even maintaining separate ticket booths.
New York Downtown has been affiliated with the NewYork-Presbyterian health care system while maintaining separate operations.
In the go-go management language of the era, L.A. and D.C. spotted a "market inefficiency" in their longtime practice of maintaining separate A-lists.
More than one million couples in the UK are squandering £5bn a year by maintaining separate homes, according to research published by Santander Mortgages today.
With Facebook and Google+ insisting on real names to maximise revenue, maintaining separate identities online for different aspects of your life isn't necessarily straight-forward.
Since 1983, The Seattle Times and The Post-Intelligencer have had a joint operating agreement, maintaining separate, competitive newsrooms while sharing most other expenses.
Maintaining separate rules makes those exceptions easier to ignore; it makes it easier to think of women's athletics as secondary, deserving of less attention and less money.
For most people, Wilcox recommends maintaining separate bank accounts while also opening a joint account used to pay common bills.
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