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Also described are the designs of a 25‐L glass bioreactor, a mechanical cell homogenizer, a device for inserting short EPR tubes into the standard Oxford Instruments EPR cryostat, and a device for transferring samples from Mössbauer holders to EPR tubes while maintaining samples at liquid N2 temperatures.
It is essential to grind the tissue as finely as possible, maintaining samples as cold as possible during grinding to avoid degradation.
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Long term immersion tests showed that the coating was effective in maintaining sample bulk integrity for the duration of the 30 day tests.
The key to this flexibility is the scalable and modular software infrastructure, providing a comprehensive solution to sample management, and satisfying the need to improve throughput while maintaining sample integrity and inventory accuracy.
Rather than the usual practice of increasing sample size with a less positive interim outcome, here we propose maintaining sample size with such a result and wait for fully mature data.
Model predictions, relating the sample separation time to a chamber taper angle, indicate respective separation times of 37 28 s for a chamber with a taper angle ranging from 16 to 20°, while maintaining sample volumes in the 1 1.5 ml range.
The thermal drill has the advantage of simplicity of design and operations and the ability to penetrate ice over a range of densities and hardness while maintaining sample integrity.
The taxonomic prey categories were chosen to achieve maximum prey resolution, whilst maintaining sample size.
The choice of prey categorisation used in the analyses was a pragmatic one, and a compromise between achieving sufficient prey resolution to identify diet changes, and maintaining sample sizes.
For maintaining sample quality, the procedure was specified by means of a standard operating procedure.
Actual segments were defined to be more contiguous in an attempt to maximize homogeneity, localize environmental exposures, and increase operational efficiency while maintaining sampling design requirements.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com