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These findings further support a role for mTOR signalling in maintaining protein stability in misfolding disease.
Altogether, our strategy innovatively addresses the roles of solvated cavities in maintaining protein stability and function.
Several successful GPCR structure determination efforts have shown that the addition of cholesterol analogs is often critical for maintaining protein stability.
Thus, it is demonstrated that the "turn" (Gln269-Arg277) betwoen two helices may be important in maintaining protein stability and formation of bioactive dimer.
One limitation of this technique, however, is maintaining protein stability and activity during the fabrication process.
For instance, maintaining protein stability usually involves interactions between conserved residues at the core of the structure.
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What distinguishes P. halocryophilus Or1 is that only three of the five indicators show significant cold adaptation, which is likely related to its broad growth temperature range where 'hot-adapted' proteins would also be required to maintain protein stability and sustain growth at its upper limit of 37 °C.
However, due to the module's diverse physicochemical properties, modularisation of capsomeres and VLPs requires tailoring of the physicochemical environment specific to each module to maintain protein stability against aggregation.
N-Linked oligosaccharides can serve as a signal for intracellular sorting and cell cell interactions, participate in protein folding and trafficking, promote resistance to proteases, prevent protein aggregation, and/or maintain protein stability.
Slp1 has three N-glycosylation sites and that simultaneous N-glycosylation of each site by the α-1,3-mannosyltransferase Alg3 was found to be required to maintain protein stability and the chitin-binding activity of Slp1, which are essential for its effector function (Chen et al. 2014).
The results highlight the importance of disulfide bridges in a small bioactive peptide to bring together frustrated structure in addition to maintaining protein structural stability.
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