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To further highlight the importance of L29 in maintaining protein expression, we used mutagenesis and selection to obtain L29 variants that enhanced GFP expression.
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In samples with PTEN allelic loss 41% maintained protein expression.
As the fiber cells mature, they lose the ability to maintain protein expression and turnover since the organelles are degraded.
This would lead to the view that negative self regulation enables homeostatic control; the cell can use negative self regulation to maintain protein expression at a desired level [ 11- 13].
The introduction of mesopores enhanced the expression of ALP and BSP in the cells grown on the mesoporous HA coatings, on the premise of maintaining the protein expression in a sequence to ensure the correct temporo-spatial expression in osteogenesis.
Several C-terminal fusions were tested, but only those that maintained detectable protein expression were capable of an NIa-dependent HR.
Proliferating progeny of satellite cells, myoblasts, maintain Pax7 protein expression and upregulate the expression of MyoD, a muscle specific transcription factor.
The results demonstrated that periostin physically associated with the Notch1 precursor to maintain its protein expression level and affected the subsequent Notch signaling under a stress condition.
hMSCs expressing SYT-SSX1 (hMSCSYT-SSX1) displayed a survival rate comparable to that of wt cells and maintained fusion protein expression for more than 3 months (data not shown).
Different from this previous report, we propose that the Notch1 protein level is decreased under the oxidative stress condition but that periostin physically associates with the Notch1 precursor, and then maintains Notch1 protein expression and subsequent signaling under this stress condition.
The mechanistic investigation further revealed that p27 maintained PHLPP protein expression, which hampered Ras activation.
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