Shi, X., Gipp, J. & Bushman, W. Anchorage-independent culture maintains prostate stem cells.
We propose that this mechanism may account for maintained prostate cancer cell growth through concerted activity with the AR in HES6-driven androgen insensitivity, where the AR persistently binds to chromatin at a subset of binding sites and seems to be essential for the survival of the resistant cells, which cannot withstand AR knock-down.
We assume that prostate cells maintain a constant concentration of total AR (the sum of bound and unbound AR).
Based on the observation that the descriptive epidemiology of prostate cancer resembles that of vitamin D insufficiency, Schwartz and Hulka (1990) proposed that vitamin D maintained the differentiated phenotype of prostate cells and that low levels of vitamin D increase the risk for prostate cancer.
Adult prostate stem cell biology is highly analogous to the biology of the developing prostate from embryonic prostate stem cells, and understanding the pathways that govern normal prostate development lends insight into the pathways that maintain adult prostate stem cells and prostate cancer stem/progenitor cells.
It seems counterintuitive that prostate cells would dedicate extra resources to maintain BAD phosphorylation instead of eliminating its expression.
Theoretically, only low levels of E1 expression are required to initiate and maintain the continuing viral oncolytic cycle to eradicate all prostate cells inside a tumor.
The homogenate suspensions were maintained in culture in collagen-coated plates with a specific medium that allowed the propagation of primary prostate cells BRFF-HPC11 medium, AthenaES, Baltimore, MD).
For example, the prostate-specific membrane antigen, which attaches only to prostate cells, could be used in this way to treat prostate cancer.
Prostate specific antigen (PSA) is a protein made by prostate cells.
