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Maintaining high specificity and consistent sensitivity across diverse HIV strains is critical for these assays to accurately detect HIV infection and expedite delivery of patient results.
While a handful of the individual residues that we identified as critical have previously been postulated to be important for TAS2R signaling (discussed below), our work extends these earlier results to explain how the TAS2R binding pocket accommodates structural diversity while maintaining high specificity.
A cutoff value of 2.70 µg/ml identified a modest subset of ALS patients (sensitivity: 32%) while maintaining high specificity versus controls (specificity: 78% vs. all study controls, 100% vs. mimic disease controls).
These autocatalytic strategies have resulted in unmatched sensitivities while still maintaining high specificity.
Next, a large amount of DNA (10 30 μg/25 μl) can be synthesized in a short time (15 60 min) while maintaining high specificity.
This detection method effectively utilizes the characteristics of the LAMP method, i.e., a large amount of amplification product can be synthesized in a short time while maintaining high specificity.
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SYBR green I dye simplifies the experimental design but introduces the need for specific controls to maintain high specificity.
We utilised highly specific hybridisation probes and primers to maintain high specificity for target genes and thereby reduce false positive outcomes.
The specific aim of this study was to identify a BBN analogue that can be radiolabeled with 177Lu and maintains high specificity for GRPr positive prostate cancer tumors in vivo.
To maintain high specificity and allow probe binding and hydrolysis of a probe with low melting temperature, a two-stage touchdown PCR was designed with a first round of amplification at high temperature and a subsequent round at low temperature to allow accumulation of fluorescence.
The use of a stringent definition of a positive result was implemented in this study in order to maintain high specificity [13].
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com