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The macromer solution containing cells can be injected in a minimally invasive manner into a defect site and crosslinked while maintaining high cell viability.
Since these modifications also reduce the biocompatibility of resulting hydrogels, we investigated a series of biocompatible buffers and crosslinkers to decelerate gelation while maintaining high cell viability.
We harness this behavior for high-throughput, convective intracellular delivery of large polysaccharides (2000 kDa), particles (100 nm), and plasmids while maintaining high cell viability.
During this cultivation process, the aggregation of cell-laden microcarriers was apparent, which resulted in aggregates of 700 800 μm, a size permissive for maintaining high cell viability.
These data suggest that the normal processes of the cells were affected less when cryopreserved using PP-50/trehalose than Me2SO, while maintaining high cell recovery.
Analysis revealed that NanoScript induces transcription and overexpresses the reporter plasmid by 15-fold in comparison to unmodified AuNPs, while maintaining high cell viability.
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Generally glasses with 5 15 mol.% TiO2 supported MG63 cell growth and maintained high cell viability for up to 7 days culture, which is comparable to Thermanox®.
Moreover, bioreactor-associated preconditioning of the SMC sheet-combined vascular scaffold maintained high cell viability (95.9 ± 2.7%) and phenotypes and improved cellular infiltration and mechanical properties (35.7% of tensile strength, 47.5% of elasticity, and 113.2% of elongation at break).
In order to remove templates efficiently for the construction of 3D interconnected macropores and to maintain high cell viability during the template removal process using EDTA solution, various factors in fabrication, including alginate viscosity, alginate concentration, alginate microbeads size, crosslinking calcium concentration, and gelatin network density were investigated.
On the other hand, NIH/3T3 mouse fibroblasts incubated with the same concentration of NO/THCPSi NPs for 48 h maintained high cell viability.
Although the continuous fed-batch strategy based on complex medium (B2) led to the highest volumetric enzyme activity, superior production costs related to the large amount of reagents required to achieve and maintain high cell densities restricted its economic performance.
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maintaining endothelial cell
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maintaining 'normal cell
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maintaining high output
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maintaining high tobacco
maintaining normal cell
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