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However, the reconstruction of these complex biosynthetic schemes faces numerous challenges due to the number of enzymatic steps and challenging enzyme classes associated with these pathways, which can lead to issues in metabolic load, pathway specificity, and maintaining flux to desired products.
In contrast, S. coelicolor encodes another UrtR homologue, TamR, which is responsible for regulating the activity of genes encoding proteins involved in maintaining flux through the citric acid cycle: DNA binding by TamR is attenuated by trans-aconitate and closely related compounds but not by urate.
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The corresponding inward convection of flux tubes must have been elevated to maintain flux conservation.
The macro equivalent current density J, which is composed of eddy currents to maintain flux quantum beam in the numerous flux pinning centers, was enhanced with increase of the initial captured flux.
The GIMME (Gene Inactivity Moderated by Metabolism and Expression) algorithm, proposed by Becker and Palsson [ 14], maintains flux through a proposed metabolic objective (similar to FBA) by simultaneously punishing flux through unexpressed reactions.
It is also possible that the catabolic pathways activated upon sucrose starvation at 80 and 100 h were suppressed in elicitor-treated cells to maintain flux into secondary metabolism.
It is of note that although FA biosynthesis enzymes were transcriptionally downregulated, their absolute abundance remained relatively high at this stage, ranging from a minimum value of 3 FPKM for KAS3 to 572 FPKM for ACP2, presumably to maintain flux towards FA biosynthesis (Fig. 2c).
Enrichment in foam fractionation is enhanced by reducing the liquid flux while maintaining the flux of bubble surfaces.
Engineering P. putida S12 for phenol production has yielded a strain that channels carbon fluxes to previously unfavourable routes to reconcile the drain on metabolites required for phenol production, while maintaining basal flux levels through central carbon metabolism.
But in microaerobic conditions, the limited oxygen converts NADH produced during cell growth into NAD+ while maintaining carbon flux into ethanol production.
By maintaining NO flux on a similar scale to endogenous endothelial cells, implanted glucose sensors exhibited reduced surface clot formation which enables more accurate quantitative glucose measurements continuously.
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