Similar to endothelial cells, epithelial cells' AJs contribute to maintaining epithelial barrier integrity (Mezzano et al., 2014).
p63 is critical for maintaining epithelial development and morphogenesis (Senoo et al., 2007).
E-cadherin (epithelial cadherin) is essential for maintaining epithelial integrity of many embryonic and adult tissues.
It serves as an adherent molecule to maintain epithelial phenotype of cell thereby inhibiting cell invasiveness.
Epithelial cadherin (E-cadherin) is the major molecule resided within epithelial adherent junctions.
Acanthamoeba castellanii (ATCC 30234) was maintained as adherent cells in PYG media in 75 cm2 tissue culture flasks at 25°C [28].
HEK293 cells (ATCC) were maintained in adherent culture in DMEM F12 supplemented with 5% FBS.
The same growth media used to maintain monolayer cultures (see Materials and methods) were used to prepare the cells and maintain the adherent and non-adherent cells in culture.
The cells were maintained as adherent cultures in retinal differentiation medium (RDM) until the appearance of pigmented RPE cells.
PAX6-depleted CECs maintained the epithelial morphology, but became larger.
