As typical of retrospective studies, our study is limited by biases such as lack of random assignment, patient selection and incomplete data acquisition, although prospectively maintained data bases were extracted.
Hence, the results of our study are shortened by a lack of random assignment, patient selection, and incomplete data acquisition even though prospectively maintained data bases were extracted.
As a part of the effort to maintain a high data acquisition and reduction rate, we have developed a Matlab-based software that acts as an interface between the user and the high performance computing (HPC) cluster.
Fifteen minutes after administration of the anaesthetic, the mice were placed on a custom-made heat block to maintain body temperature during data acquisition.
HL was pivotal in the data acquisition and maintaining accuracy of the analysis, interpretation and writing of the manuscript.
Temperature of the probe was maintained at 27 °C during the data acquisition.
Once inside the XPD stream (position 1, Figure 1C), the tension was increased to a fixed value below the hairpin unfolding force and maintained using force feedback, and data acquisition started.
In the infusion group, a continuous central venous infusion of Pseudomonas aeruginosa (strain O1 isolated from a patient with suppurative otitis, 1 × 109 colony-forming units/ml determined serial dilution and colony counts) was commenced after baseline data acquisition and maintained until the end of the study.
Repeated acquisition on the same precursor ion and short exclusion times were chosen to maintain sufficient redundancy for MS/MS data acquisition providing higher quality data.
The electron dose rate applied onto the Mimivirus sample over the data acquisition course was maintained at a low level, within 2 3 electrons per Angstrom square per second.
