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In DMEMpr−, both tissue architecture and cellular heterogeneity were much better preserved: epithelial cells showed a more columnar shape, and stroma was very well conserved, maintaining cell density.
Cultures were incubated for about 4 days at 37°C, 5% CO2 atmosphere, then disaggregated and counted daily, maintaining cell density between 0.2 and 1 × 10 cells per ml.
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Cells were passaged every 2 3 days maintaining cell densities between 0.5 2 × 10 cells/ml.
Some of these questions require new techniques to be developed in order to address them, including methods to culture cells in microfluidic chambers that maintain cell density and communication, reporters that more accurately reproduce the actual behavior of the genes they represent, as well as tracking individual cells within the chemostat over time.
Cultures were split in two to maintain cell density below 1 × 10/mL.
Parasites were counted every 24 hr using a Coulter Counter (Beckman Coulter, Brea, CA), and cultures were diluted to maintain cell density between 10 and 2 × 10 cells/ml.
(2012) develop a culture method that overcomes current limitations in ex vivo hematopoietic stem/progenitor cell expansion by continuously diluting inhibitory signaling factors and maintaining stem cell density.
Normal epithelial cells produce S1P to trigger their extrusion and death once they become too crowded, simultaneously maintaining correct cell density and barrier function.
Human lymphoblastoid cells were maintained in RPMI 1640 with 10% FBS, 2 mM glutamine, 1× penicillin/streptomycin solution and were maintained at cell density of 0.3×10 2×10 cells per ml.
How does a population of cells in a multicellular organism maintain its cell density?
Therefore, to maintain sufficient cell density for SrtA expression, fermentation at 30 °C for 8 h (the second highest SrtA activity) was chosen as the optimal condition in the following experiments.
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