In the current work, we focused on evaluating the changes in technical variability and the effect of different normalization pipelines, which should mainly consist in eliminating or reducing the variability introduced during the experimental process, while maintaining the biological variation between conditions.
Correction with either ComBat or XPN reduced variation to a level similar to that seen in either dataset independently, further suggesting that gene-wise cross-platform bias is reduced, while true biological variation is maintained (Additional File 2D).
Rodents, such as mice and rats, have the advantage of being purpose bred to reduce biological variation, while being affordable, and easy to breed and maintain in-house.
Speed is obviously of great importance, but equally important is maintaining alignment accuracy of short reads, in the 25 100 base range, in the presence of errors and true biological variation.
Evolutionary theory has been successfully developed as a tool for investigating the ultimate causation of biological variation in multicellular organisms, and is increasingly being applied to explain why genetic variation is maintained for traits, such as virulence, in unicellular parasites.
Attention to the complexities of biological variation is growing.
Mt. Ararat — where, in Noah's time, the densest collection of biological variation once confronted the direst environmental peril — hulks symbolically in the distance.
Biological variation in penetrating the skin was shown.
Available data on biological variation of HbA1c revealed marked heterogeneity.
But biological variation makes doing so difficult.
