Sentence examples for maintaining and treating from inspiring English sources

After maintaining and treating the cells as indicated, cold PBS was immediately added, plates were washed twice in cold PBS and adherent cells were lysed in CelLytic lysis buffer (Sigma) containing protease inhibitors (Roche, Indianapolis, IN, USA).

Studies suggests that rainwater collection systems which are properly designed, maintained, and treated may provide a valuable supplement to existing water supplies by reducing demand on community water supplies/infrastructure costs, enhancing effective management of storm water runoff, and increasing restoration of underground reservoirs through controlled infiltration.

The animals were maintained and treated in compliance with the guidelines of the Swiss Veterinary Office and the Cantonal Veterinary office of Basel-Stadt (approval 2085 and 789).

All cells were maintained and treated at 37° and 5% CO2 unless otherwise noted.

Animals were maintained and treated according to the Institutional Animal Care and Use Committee at the Tel Aviv University.

Animals were maintained and treated according to the guidelines of animal welfare.

PrEC cells were maintained and treated in PrEGM (Lonza, Mt Waverley, Vic, AU), and transfected in PrEGM without antibiotics.

All mice used for the experiments were 6 10 wk old females and were maintained and treated in accordance with the guidelines of the Animal Use Committee at LSU Health Sciences Center.

For organotypic striatal slice cultures, brain slices harvested from treatment-naïve rats were maintained and treated in vitro as described below.

All mice were maintained and treated under the Home Office's animals (scientific procedures) Act 1986 under the Home Office approved Project Licence 70/5267.

Mice were bred in the Weizmann Institute of Science (Rehovot, Israel) and maintained and treated according to NIH guidelines.