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In all cases, the reaction mixtures were supplemented with β-glucosidase to avoid inhibition by product, maintaining a final enzyme concentration at 34 mM.
The investigations revealed that the best conditions for the reaction were found by maintaining a final concentration of 0.0025% w/v of FLC, a neutral pH using simple water as diluting solvent and leaving the reaction to proceed for five minutes at room temperature.
A lab-scale reaction was conducted using a 100 μL reaction volume and maintaining a final concentration of 50 mM Tris HCl buffer at pH 7.4, 10 mM MgCl2 6H2O, 2 mM substrate (dissolved in Dimethyl sulfoxide (DMSO)) and 4 mM NDP-sugars individually with 30 μg/mL of appropriately diluted enzyme, and the remaining volume was Milli-Q distilled water.
Trametinib (LC Laboratories) was added from a 62.4 mM stock solution in DMSO maintaining a final DMSO concentration of 0.25% (v/v).
The total number of added nucleotides was 702, thus maintaining a final genome length that was an even multiple of six, as required for efficient RNA replication (Kolakofsky et al. 2005).
Similar(54)
British Airways is typical in contributing the equivalent of 25%to50%0% of workers' salaries into its pension scheme to keep pace with the growing costs of maintaining a final-salary guarantee.
My choice was to take 25percentt a year off the table for the past three years, but to maintain a final, 25percentt position in the market.
The government seems least likely to maintain a final set of benefits — leniencies in loan terms that taxpayers effectively have subsidized for borrowers.
Even as they put their lives on display, she thought, artists thrive when they maintain a final redoubt of privacy — a wellspring that remains unpolluted by the world outside.
The production team was responsible for casting, but Microsoft maintained a final say.
The third phase corresponded to the final position, where the subject had to maintain a final upright standing posture, keeping the left arm raised horizontally.
More suggestions(15)
update a final
maintaining a golden
maintaining a sanctuary
maintaining a strong
maintaining a perfect
maintaining a short
maintaining a daunting
maintaining a creative
maintaining a comfortable
maintaining a great
maintaining a precise
maintaining a vibrant
maintaining a good
maintaining a clean
maintaining a long
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Justyna Jupowicz-Kozak
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