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However, pollen produced by the F1 (sterile × maintainer) plants was sterile.
Pol CMS and maintainer plants of non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino) were grown in a greenhouse under a light/dark cycle of 16 h light/8 h dark at 24/16°C.
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The maintainer F1 sterile plants and the fertility restorer F1 plants were subjected to RT-PCR analysis using the 5' gene specific Mtg-1 and the 3' gene specific Corf primers.
The ~770 bp RT-PCR products were cloned and for maintainer and restorer plants, 15 randomly selected clones were sequenced.
Northern blot analysis revealed the presence of two bands, a ~0.7 kb and a longer ~1.1 kb band in the maintainer and restorer F1 plants.
In maintainer and restorer hybrid plant lines, the two orfB transcripts were present; but the ~1.1 kb transcript was edited in the restorer hybrid lines.
Thirty-two clofes of the maintainer F1 APMS-6A × APMS-6B) plants were randomly selected and sequenced and provided evidence for the presence of edited and unedited sequences.
Regenerated maintainer lines of stem mustard plants from medium containing kanamycin (50 mg/L) were treated as putative candidates and were further screened by rooting them on medium containing kanamycin (25 mg/L).
The genotype is identified to be rf17rf17 for a plant in lane 1, and Rf17rf17 for a plant in lane 2 Fig. 2 Maintainer line (IR24), CMS line of IR24 (CWA x IR24 BC6F1) and restorer line of IR24 (CWR x IR24, BC4F1).
The influence of the nuclear encoded Rf alleles on transcription of the orfB gene was tested in two types of F1 plants, sexual hybrids APMS-6A × APMS-6B (maintainer) and APMS-6A × BR-1870 (restorer).
Proline, an amino acid known to function as an osmoprotectant, a ROS scavenger, a cell structure maintainer, and a signaling molecule, plays an active role in plant stress responses.
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