Sentence examples for maintained with free from inspiring English sources

Wistar albino mice were procured from Central Animal House, Rajah Muthiah Medical College, Annamalai University, India and housed in the Institutional animal house under standard environmental conditions (22 ± 3°C, 55 ± 5% humidity and 12 h/12 h dark/light cycle) and maintained with free access to standard diet and water ad libitum.

Throughout the experiments, mice were maintained with free access to pellet food and water in plastic cages at 21±2°C and kept on a 12-hour light-dark cycle.

Animals were maintained with free access to food and water.

The animals were maintained with free access to standard diet and tap water.

Animals were maintained with free access to water and food in an air-conditioned room on a 12-h light dark cycle.

Animals fed ad libitum were maintained with free food access for 3 weeks; on the last day, food was removed at 14 00 h, and, after 21 h (~1 day) of food deprivation, they were sacrificed (at 11 00 h), F group.

When cells achieved the desired density of > 80% confluency original medium was removed and cells were maintained with serum-free media for 14 h.

The neurons were subsequently maintained with serum-free NeuroBasal medium (Gibco, Grand Island, NY, USA) containing 5 mM glucose, and used at day 10 12 in vitro.

Animals were maintained with specific pathogen free air at a temperature between 20 and 23°C with 12-h light and dark cycles and relative humidity of 50%.

All mice were housed in pathogen-free conditions and maintained with 12 h dark/light cycle with free access to food and water.

Whether in the household hearth or, at a later period, in fire temples, the sacred fire had to be maintained with proper fuel, kept free from polluting agents, and above all never permitted to go out or be extinguished.