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Field experiments showed a dramatic decline in the fluorescence yield with rising light intensity in both drying and artificially maintained wet plots.
To overcome our inability to measure CO2 or O2 exchange in the natural crusts, we used artificial crusts where Microcoleus suspensions were soaked onto nitrocellulose filters (see [15]) that were maintained wet during exposure to 500 µmol photons m−2s−1 of blue and orange light.
Crusts were formed following incubation (25°C, 30 µmole photons m−2 s−1) in Petri dishes on filter paper maintained wet by periodic additions of water, and continued to grow in thickness for up 48 h (about 0.1 0.2 mm thickness in the wet state).
The articular surface was maintained wet with PBS during the embedding process.
After light treatment, the thalli were maintained wet in darkness (to allow V-cycle re-epoxidation) for another 60 min. Samples were incubated in the dark for 24 h in glass hermetic chambers, containing a NaCl saturated solution (75% Relative Humidity -RH-).
For microscopic observations, the fibre scaffolds are either transferred to glass slides, maintained wet and observed with standard non-immersion objectives (without the addition of a cover slip); or for imaging at longer working distances, they are submersed in fresh growth medium, followed by imaging using a water-dipping objective.
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Eggs were maintained on wet peat moss at room temperature in sealed Petri dishes.
Third, it is durable, robust and portable, as the sliced and packaged Genopal™ microarrays are maintained under wet conditions.
The oligonucleotides embedded in Genopal™ are maintained in wet conditions throughout all steps of the experiment, even during scanning, thereby allowing for rapid and easy microarray assay.
Experimental flies bearing the 109-30-Gal4 109-30-Gal4 109-30-Gal4t 25°constructn shifted to 29°C 1–2 days post eclosion and maintained on wereyeast.
For follicle cell experiments, freshly eclosed females were maintained on wet yeast paste for 24 hours prior to ovary dissection, and individual stage 11 13 egg chambers were hand dissected following fixation.
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