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All were maintained under uniform conditions of feeding and drinking ad libitum, at a temperature of approximately 33°C and 24-hour illumination.
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Medaka in the present study were maintained under standard laboratory conditions at constant temperature (27±1°C) and with a uniform light:dark cycle; they do not undergo a period of winter dormancy as they would in their natural environment.
The goat was maintained under general anesthesia.
Mice were maintained under sterile conditions.
All mice were maintained under SPF conditions.
Animals were maintained under local vivarium conditions.
Samples were maintained under natural light conditions.
The MCS is maintained under negative pressure.
The test tubes were maintained under illumination.
Control endothelial cells were maintained under normoxia.
Cells were maintained under puromycin (Sigma) selection.
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