High-performance liquid chromatography analysis of the drug in this vehicle at various time points revealed that 667 COUMATE is stable when maintained under these conditions.
Fold LDH release was expressed relative to the corresponding stable cell line maintained under normoxic conditions (Fig. 7B).
Stable cell lines were maintained under 400 μg/ml G418 antibiotic selection for three weeks.
The resulting solutions of the selenols were stable for considerable periods when maintained under anoxic conditions, but rapidly re-oxidized to the diselenide in the presence of O2.
To maintain stable transgene expression, cell lines were maintained under selective pressure using 500 μg/ml of active Geneticin (G418-sulfate, Invitrogen).
Stable PTGER4 transfectant cells (PTGER4 cells) were maintained under the same conditions with the addition of a maintenance dose of 200 µg/ml G418 (Calbiochem, UK).
Stable transfectants derived from them (described below) were maintained under similar conditions except that the appropriate antibiotics were added to the media.
The expression levels of PD2 and ALDH1 were stable up to the third cell passage when maintained under stem cell-specific conditions (data not shown).
The stable cell line 293-hTLR3-HA 293-hTLR3-HA 293-hTLR3-HAm InvivoGen®, was maintained under the same conditions and cultured in regular DMEM supplemented with 10 μg/ml blasticidin [ 26].
To ensure stable overexpression of the efflux pumps in vitro, multidrug-resistant cells have to be maintained under continuous selective pressure.
