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In natural C. elegans selfing is the predominant mode of reproduction [ 53], although in the laboratory males are maintained under several environmental and genetic contexts [ 29, 32, 54- 56].
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In some places glacial till and fluval sands and gravels are maintained under the several lava flows that form the volcanic field.
The genome sequencing project utilized the PEST strain that was established from a mix of several natural populations of the M and S forms of A. gambiae, maintained under insectary conditions for several years and exposed to bottlenecks and selections [ 65].
This culture was maintained under lab condition for several generations before starting the experiments.
These cells were maintained under G418 selection for several passages prior to being used in the experiments to ensure that the experiments were conducted with stably transduced cells.
These 20 L1 individuals were then maintained under a long photoperiod for several generations as independent colonies of "putative mutants".
While timed melatonin exposure entrains circadian rhythms of several mammalian species maintained under constant conditions [ 21, 22], there is a dearth of information about potential entraining effects of melatonin presented simultaneously with a light/dark cycle.
Dimensionally Stable Anodes embedded in garden compost and maintained under constant polarization at 0.50 V/SCE for several days progressively became covered by a microbial biofilm that gave them the capability to oxidize the organic matter contained in the compost.
The isolate DAOM-197198 has been distributed to several laboratories worldwide and maintained under in vitro conditions since 1992 (Cardenas-Flores et al. 2010) and its nuclear genome has been sequenced (Tisserant et al. 2013; Lin et al. 2014).
Differentiated epithelia formed by HPTCs can be maintained under optimized in vitro conditions in the laboratory for several weeks.
Two wExe1-infected and several uninfected isofemale lines were established from field-collected pupae, and maintained under laboratory conditions for >4 generations.
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