Sentence examples for maintained under selective pressures from inspiring English sources
SUG identification within most bacterial classes indicates that SUG sequences are maintained under selective pressures that differ from other SMR homologues within their hosts.
PSMR subclass members have the most specific sequence distribution among the sequenced Bacterial genomes we surveyed strongly suggesting they are maintained under selective pressures different from either the SUG or SMP subclasses (Table 1).
Clones were maintained under selective pressure and selected on the basis of their FL1-fluorescence by flow cytometry (Gallios, Beckman Coulter).
Since this value exceeds 1.0, it indicates that SMP homologues are maintained under selective pressure within the examined microorganisms.
To maintain stable transgene expression, cell lines were maintained under selective pressure using 500 μg/ml of active Geneticin (G418-sulfate, Invitrogen).
We confirm, based on a separate, unbiased dataset, that CNC sequences are constrained, and that human-chicken conserved sequences, which evolved early in the vertebrate lineage have been maintained under selective pressure until our recent past.
Following two weeks of selective pressure the cells were purified by fluorescence activated cell sorting and the resultant BDNF-Schwann cells and control EGFP-Schwann cells were maintained under selective conditions (200 µg/ml geneticin) and sub-cultured every 3 4 days.
To analyze their replication capacity, the chimeric GBV-B/HCV RNA transcripts were transfected into cB76.1/Huh7 cells and cells were maintained under G418 selective pressure for 3 weeks.
To ensure stable overexpression of the efflux pumps in vitro, multidrug-resistant cells have to be maintained under continuous selective pressure.
According to mean dN/dS values for all NarJ subfamily members (NarJ 0.65, TorD 0.65, DmsD 0.57, and YcdY 0.24) all members were maintained under purifying selective pressure commonly observed for most genes [ 52].
A U-87 MG cell line containing a stably integrated destabilized EGFP (d1EGFP) transgene was produced by transfecting U-87 cells with the 4.9-kb pd1EGFP-N1 plasmid (BD Biosciences Clontech, Palo Alto, CA) and maintained under constant selective pressure by G418 (500 μg/mL).
