Sentence examples for maintained under selective from inspiring English sources
Clones were maintained under selective pressure and selected on the basis of their FL1-fluorescence by flow cytometry (Gallios, Beckman Coulter).
Two days after transfection, cell culture was added with 500 µg/ml G418 and maintained under selective conditions for three weeks to allow plasmid integration in the IMR-32 genome; then, survived clones were isolated and expanded in 48-wells plateswithculture medium added with 400 µg/ml G418, then expanded in 24-wells and subsequently in 6-wells plates.
Following two weeks of selective pressure the cells were purified by fluorescence activated cell sorting and the resultant BDNF-Schwann cells and control EGFP-Schwann cells were maintained under selective conditions (200 µg/ml geneticin) and sub-cultured every 3 4 days.
Since this value exceeds 1.0, it indicates that SMP homologues are maintained under selective pressure within the examined microorganisms.
SUG identification within most bacterial classes indicates that SUG sequences are maintained under selective pressures that differ from other SMR homologues within their hosts.
To maintain stable transgene expression, cell lines were maintained under selective pressure using 500 μg/ml of active Geneticin (G418-sulfate, Invitrogen).
To analyze their replication capacity, the chimeric GBV-B/HCV RNA transcripts were transfected into cB76.1/Huh7 cells and cells were maintained under G418 selective pressure for 3 weeks.
To ensure stable overexpression of the efflux pumps in vitro, multidrug-resistant cells have to be maintained under continuous selective pressure.
These new (E-plus -helpers (Fig. 5a) wE-plus -helpers into mE-plus -helpersells (by mobilization or electroporation) and maintained under strictly selective conditions without expressing the Mu transposition Figtors.
According to mean dN/dS values for all NarJ subfamily members (NarJ 0.65, TorD 0.65, DmsD 0.57, and YcdY 0.24) all members were maintained under purifying selective pressure commonly observed for most genes [ 52].
A U-87 MG cell line containing a stably integrated destabilized EGFP (d1EGFP) transgene was produced by transfecting U-87 cells with the 4.9-kb pd1EGFP-N1 plasmid (BD Biosciences Clontech, Palo Alto, CA) and maintained under constant selective pressure by G418 (500 μg/mL).
