Sentence examples for maintained under selection for from inspiring English sources
These cells were maintained under selection for approximately three weeks, at which point individual clones were isolated and cultured in single wells of a 24 well plate.
A second population of hTERT-transfected fibroblasts was also made, which we maintained under selection for 20 weeks.
In contrast, our protocol for additive gene transfer uses pools of mixed cell clones, which have been maintained under selection for 7 to 10 days [ 8, 18, 41].
With the exception of primary class 1 tumor cells, which were under selection for one week, all infected cells were selected for at least two weeks before use in experiments and were maintained under selection for up to four weeks (referred to as BAP1-deficient or control stable cells).
MC3T3 cells were transfected at 70-80% confluence with FuGENE® (Roche) following manufacturer's specifications, selected with 0.5 µg/ml puromycin, and maintained under selection medium for the duration of the experiments.
After 2 days, puromycin (100 ng/ml) was used for selection and cells were maintained under selection until single cell clones were obtained by limiting dilution.
oriP is also a paradigm for a mammalian autonomously replicating sequence (ARS), which can be efficiently introduced and maintained under selection in cells expressing EBNA1.
Firefly luciferase-expressing 4T1 (4T1-luc) cells were maintained under selection in G418 (400 μg/ml; Calbiochem).
These cells were maintained under G418 selection for several passages prior to being used in the experiments to ensure that the experiments were conducted with stably transduced cells.
The cells were grown for an additional 48 hours after transduction and then maintained under puromycin selection (4 μg/ml) for five days.
Finally, analysis of CRR21 genes in sequenced genomes that have a genomic T at ndhD C383 demonstrates that CRR21 truncation is observed in three additional species of the Malpighiales, and that these genes are maintained under strong negative selection for what appears to be an extended period of evolution.
