Sentence examples for maintained under selection from inspiring English sources

MC3T3 cells were transfected at 70-80% confluence with FuGENE® (Roche) following manufacturer's specifications, selected with 0.5 µg/ml puromycin, and maintained under selection medium for the duration of the experiments.

These cells were maintained under selection for approximately three weeks, at which point individual clones were isolated and cultured in single wells of a 24 well plate.

Cells are grown in Dulbecco's minimal Eagle's medium (DMEM) supplemented with 10% FBS and 2.5 mM L-glutamine and maintained under selection with 40 µg/ml hygromycin at 37°C in 95% air/5% CO2.

A second population of hTERT-transfected fibroblasts was also made, which we maintained under selection for 20 weeks.

Firefly luciferase-expressing 4T1 (4T1-luc) cells were maintained under selection in G418 (400 μg/ml; Calbiochem).

After 2 days, puromycin (100 ng/ml) was used for selection and cells were maintained under selection until single cell clones were obtained by limiting dilution.

ALHF is a wild-type strain collected from a poultry farm in Alabama in 1998, selected with permethrin for 6 generations, achieving a 6,600-fold 6,600-fold, and maintained underesistance selection with permethrin [ 25, 26].

However, prion diseased brain contains a highly heterogeneous population of abnormal PrP isoforms that may differ with respect to their conformation, aggregate size, potential neurotoxicity and specific prion infectivity [5] and indeed prion strains, rather than constituting a molecular clone, appear to comprise an ensemble or quasispecies maintained under host selection pressure [5], [37].

This strain has been maintained under biannual selection with permethrin.

Colonies were picked and maintained under G418 selection.

MC38 CEA) cells were maintained under antibiotic selection pressure from Geneticin (0.5 mg/mL).