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Timed-pregnant Long-Evans rats (body weight ~ 230 g; Charles River Laboratories, Portage, MI) were obtained on gestational day (GD) 3 (day of insemination is GD0) and were housed individually in standard polycarbonate plastic cages containing heat-treated pine shavings as bedding and maintained under regulated temperature (21 ± 2°C), relative humidity (50 ± 10%), and a 12-hr light/dark cycle.
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The repressed chromatin state of the C. elegans X chromosomes is interspersed in cis over short or long distances that is maintained under normal growth and development conditions, thus regulating the global expression of the X chromosomes (Fong et al., 2002; Kelly et al., 2002).
The goat was maintained under general anesthesia.
Animals were maintained under local vivarium conditions.
Samples were maintained under natural light conditions.
Mice were maintained under sterile conditions.
All mice were maintained under SPF conditions.
The MCS is maintained under negative pressure.
The test tubes were maintained under illumination.
The leaves were maintained under ambient conditions.
Animals were maintained under UK Home Office Project Licence no.
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