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The sampled leaves were stored at 1.5 mL microtubes containing CTAB/salt gel (3 % CTAB, 35 % NaCl), and maintained under refrigeration at 4 °C for, at least, 7 days before DNA extraction.
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Although the use of viral transport medium should ensure that virus will maintain viability under refrigeration, we pre-tested the effect of transportation procedures on virus viability by using a sample of viable human influenza virus in virus transport medium (provided by ProvLab) in 2 pre-tests (including courier to Memphis and culture in Memphis).
Mice were maintained under sterile conditions.
All mice were maintained under SPF conditions.
Animals were maintained under local vivarium conditions.
Samples were maintained under natural light conditions.
Previous studies have shown that glucosinolate levels can be maintained by refrigeration [ 42] or exposure to radiation [ 43]; here we find that post-harvest storage under light/dark cycles can also lead to sustained glucosinolate levels.
It will keep four weeks under refrigeration, six months in the freezer and is soft enough to be spooned out while frozen.
In the meantime, Bohanan says that investigators should dust for children's fingerprints immediately at a crime scene and keep key evidence under refrigeration.
And food banks have their own operating expenses, from maintaining refrigeration rooms to fueling trucks and vans that deliver groceries to charities.
"If the oven goes, you can't cook, but you don't lose your entire inventory," said Bob Levine, the president of Americold, which installs and maintains refrigeration units.
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