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Hypothermia and alkalinization combined (20 °C/pH 9.3) provided the greatest neuroprotection with a yield of viable neurons after 1 h of ischemia 2.5-fold larger than that from DRG maintained under physiological conditions (37 °C/pH 7.6).
Hypothermia (20 °C>15 °C>37 °C) and alkalinization (pH 9.3>pH 8.3>pH 7.4) increased the yield of viable neurons compared with the yield from DRG maintained under physiological conditions.
All confocal images shown are of living embryos maintained under physiological conditions.
These analyses indicate that only mouse cells maintained under physiological O2 concentrations develop a SASP that is quantitatively and qualitatively similar to the human SASP.
We concluded that adult mice maintained under physiological conditions but lacking Sox6 in erythroid cells exhibited compensated anemia, with underdevelopment of proerythroblasts, impaired maturation of erythroblasts and reticulocytes, and decreased survival of RBCs.
Several studies conducted with embryonic stem (ES) cells reported that characteristic stem cell properties are preserved only when ES cells are maintained under physiological O2- ES cells otherwise differentiate under ambient O2 as reviewed in [2].
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We conclude that the previously established oxidant selectivity is highly robust and likely to be maintained under most physiological conditions.
The NAD+/NADH ratio maintained under normal physiological conditions manifests as a 10/1 lactate/pyruvate ratio in the cytosol.
Our results show that the nucleosomal profile in S. pombe is largely maintained under different physiological conditions and patterns of gene expression.
Over 4 h of ischemia, the yield of viable neurons from DRG maintained under both hypothermic/alkaline and physiological conditions decreased in a linear manner, but those at 20 °C/pH 9.3 had a 4.5-fold greater yield of viable neurons than those at 37 °C/pH 7.6.
Plants were maintained under irrigated conditions until physiological maturity at which seed samples were harvested from a pool of at least three plants per RIL for each replication.
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CEO of Professional Science Editing for Scientists @ prosciediting.com