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The fish were transferred back to Tianjin Agricultural University and maintained under optimal rearing conditions (C. carpio var. Koi and C. carpio var. Jian: 20 °C, pH 7.5; C. semilaevis: 23 °C, pH 7.5, salinity 22 ppt) for 1 week.
They were maintained under optimal conditions of 12 hr light/dark cycles, with temperature (20 ± 2°C) with relative humidity (50 ± 10%) kept constant.
Marine medaka (O. melastigma) were reared and maintained under optimal growth and breeding conditions, as described in Kong et al. (5.8 mg O2 L−1, 28 ± 2o C, pH 7.2 in a 14-h light: 10-h dark cycle) [ 1].
Given the critical role of the metabolic milieu in cancer progression, data acquired from cancer cells maintained under optimal culture conditions may have limited translational potential, findings that have been confirmed in assessments of effective drug treatments [ 85].
Such conflicting data may arise from the fact that laboratory mice are normally maintained under optimal husbandry conditions, their antioxidant defense is well balanced and works efficiently in protecting from relatively low levels of ROS, therefore overexpression of antioxidant enzymes has a marginal effect.
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We studied rapidly growing caiman juveniles maintained under constant optimal conditions, so our measured rates of incorporation probably represent higher rates for this species than those seen in wild caimans enduring episodic nutritional constraints and growing more slowly (e.g. adults).
It is confirmed that the optimal control performance is maintained under conditions of strong wind excitation and an optimally designed small blocking ratio.
However, this natural safeguard can be subverted by breeders, who are able to maintain plants under optimal growth conditions, and often need only to obtain pollen to advance to the next generation.
The goat was maintained under general anesthesia.
Mice were maintained under sterile conditions.
All mice were maintained under SPF conditions.
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