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Fold LDH release was expressed relative to the corresponding stable cell line maintained under normoxic conditions (Fig. 7B).
RNA was isolated from primary rat cultures that were highly purified (>98%) for either cerebral cortical neurons, or astrocytes that had undergone OGD or were maintained under normoxic conditions as described above.
Controls were sham-exposed and maintained under normoxic conditions.
Additionally, myoblasts cultured under hypoxic conditions show increased proliferation compared to cells maintained under normoxic conditions.
Control cultures for protein and gene expression analysis were maintained under normoxic conditions.
The control group was maintained under normoxic conditions (6.5 ± 0.2 mg O2/L).
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We measured levels of EGF-R mRNA in these transfected cells and found that H H induced a 3-fold increase in EGF-R mRNA levels in control transfected NPs versus NPs maintained under normoxic-normoglycemic conditions.
The other group (normoxic mice, Control, n = 7) was maintained under ambient normoxic conditions (FiO2 = 21%), with the same 12∶12H light-dark cycles.
To assess the effect of hypoxia on expression of CD133, we cultured U251MG human glioma cells in 1% oxygen for up to 3 days and compared them to cells maintained under standard normoxic conditions (21% oxygen).
However, before cells were exposed to the hypoxic condition, they were treated under normoxic condition for 1 h then maintained under hypoxic conditions for 16 h.
We first evaluated the positive EP under normoxic conditions when an anesthetized animal is maintained under normal tissue oxygen levels by spontaneous breathing and the negative EP under anoxic conditions when tissue oxygen levels were depleted due to cessation of spontaneous breathing (Fig. 4C and E).
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